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Although there are approximately 2,000 species of scorpions found worldwide, only a few species are considered "lethal" to humans. However, many cases of scorpion stings have been reported around the world. Scorpion bite is one of the major health problems of underdeveloped countries, in tropical and subtropical regions, which causes widespread clinical complications and sometimes death. Serum therapy is the only way to treat patients affected by scorpions. Although there is extensive information about scorpion stings and the effect of scorpion venom on public health, there is little information about the collection and creation of scorpion colonies for the purpose of extracting venom and producing antiven-om from them. So far, no study has been conducted in our country to investigate the production of anti-venom from scorpion collections kept in captivity.
In this project, old poison was extracted despite the long period of effectiveness. In this research, the quality of the old extracted poison was not significantly different from the new poison in terms of LD50, however, they were different. In terms of mortality, it was higher in the group that received the new poison. The results showed that the amount of protein in the new venom compared to the old venom had no significant statistical difference. The results showed that the amount of protein in the new venom compared to the old venom had no significant statistical difference. A slight difference in hyaluronidase enzyme activity and phospholipase enzyme activity was observed between the two poisons. For further investigation, liquid chromatography (HPLC) was also performed. Based on the results of HPLC gel chromatography, the number of peaks obtained from six species of scorpions were not significantly different between the new and old venoms. However, more peaks were observed in the new venoms of these six species of scorpions. The level of blood sugar in the laboratory animals that received the new poison was higher than those that received the old poison, but the difference was not significant. Blood biochemical factors were in such a way that in all cases these factors were more in the animals receiving the new poison than in the animals receiving the old poison. Except for the LDH factor, which was the opposite. In the case of inflammatory factors such as fibrinogen, ESR, and CRP, only in the case of fibrinogen, this value was higher in the group receiving the old poison than in the group receiving the new poison, but it was the opposite for the other two factors. Regarding hemorrhagic activity and measurement of platelet count. There was no significant difference between the old and new poison. Only its amount was slightly higher in the old poison. In general, the results obtained from the comparison of two types of poison indicated the physico-chemical difference between the new and old poison. We came to the conclusion that a more in-depth investigation and the design of separate and specialized projects on each type of scorpion is needed to be able to collect more detailed information in this field. For example, the effects of any scorpion, whether old or new, can be investigated on diabetic mice to measure blood sugar or inflammatory factors.
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